Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification     

ZHANG Fengying  SHI Yanhong  JIANG Keji  XU Zhaoli  MA Lingbo 《海洋学报(英文版)》2018,37(2):139-146

A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.  相似文献   

营养限制对三种浮游动物的影响   总被引：1，自引：0，他引：1  

陈蕾  李超伦  周孔霖  时永强  刘梦坛 《海洋学报(英文版)》2018,37(4):58-68

Nutrient imbalance—a mismatch in nutrient ratios between the available food supply and the demands of consumers—has the potential to be transported up food chains,exposing zooplankton to nutrient limitations.In this study,the response of Calanus sinicus(copepod),Moina mongolica(cladocera),and Brachionus plicatilis(rotifer) to nutrient-limited(no-limited,P-limited,and N-limited) food were evaluated from the perspective of growth,reproduction,and stoichiometric homoeostasis.The results indicated that the growth of three species was suppressed under nutrient-limited(especially P-limited) conditions.However,the effect of nutrient limitations on their reproduction was species-specific.For C.sinicus,the dietary UFA(unsaturated fatty acid) as a major phospholipid component affected their egg production and total FA supporting energy promoted the hatchability of eggs.Furthermore,excess carbon in the diet promoted egg production but reduced hatching success.For M.mongolica,nutritional(P and UFA) and energy(total FA) support affected their fecundity.B.plicatilis fecundity exhibited the same pattern of growth(no-limitedN-limitedP-limited).In terms of stoichiometric homeostasis,B.plicatilis' s elemental compositions were less likely to be affected by nutrient limitations than M.mongolica.We suggest that the effects of nutrient imbalances could potentially become an evolutionary force affecting ecosystem structure and stability in eutrophic waters.  相似文献   

长江三角洲蓝图重绘的基础科学问题:进展与未来研究     

汪亚平  贾建军  杨阳  周亮  冯威  高建华  魏稳  李占海  史本伟  高抒 《海洋科学》2019,43(10):2-12

在全球气候变化和人类活动影响加剧的背景下,作为河口海岸重要子系统的三角洲正在发生快速变化。长江三角洲地处长江入东海交汇处,是中国最重要的经济核心区之一,对邻近区域乃至整个长江经济带经济社会发展都起着重要作用。由于全球变暖、海面上升和强烈人类活动引发了三角洲系统状态转换,因此以往基于恒定系统状态而获得的有关长江三角洲的认识已不能满足未来需求,迫切需要对未来海面变化、极端事件、流域与河口工程影响下的三角洲物质循环条件、物理过程、地貌冲淤演化、源-汇格局调整等科学问题进行深入研究。在三角洲系统行为、未来演化趋势的预测能力建设中,应重视从海面到海底的综合立体观测系统的发展,以获取关键数据;基于三角洲系统的时、空演化特征,建立三角洲本征态和衍生态的谱系理论。未来需针对系统状态转换而调整原先的经济社会发展模式,以便保护自然资源、重建生态系统,更好地支撑长江经济带发展,重绘长江三角洲发展蓝图。  相似文献   

Multi-band Solar Full-disk Extreme Ultraviolet Imager     

PENG Ji-long  FENG Tao-jun  SHI En-tao  LI Lin  YU Qian  ZHANG Kai  NIE Xiang-yu  MA Zi-liang 《Chinese Astronomy and Astrophysics》2019,43(3):444-456

Solar Extreme Ultraviolet (EUV) imaging observation is an important measure for the researches of solar activities and coronal plasma physics. But the traditional EUV imager and spectrograph can hardly achieve simultaneously the high spectral resolution and wide field-of-view of solar imaging. This paper has designed a new type of solar EUV multi-band imager, by adopting a kind of slitless grating and grazing incidence structure, it can realize the solar full-disk imaging of high spectral and spatial resolution. The field-of-view of the imager can be as broad as 47′. The spectral resolution is 2×10^?3nm per pixel, and the spatial resolution is 1.4′ per pixel. The temporal resolution of the solar full-disk is better than 60 s. The analysis of the solar full-disk spectral image and system response shows that the imager can observe the morphological evolutions of various solar activities, and can provide more comprehensive data for the researches of solar physics and space weather forecast.  相似文献   

CORS系统的差分定位终端设计与实现     

刘邢巍  史俊波  蒲德祥  许超钤 《测绘通报》2020,(12):114-117

当前广泛使用的实时GNSS定位应用可分为两种：一种是低成本定位应用，其采用廉价的芯片和天线，广泛应用于手机、共享单车和公交车定位等方面；另一种是高精度GNSS定位应用，采用网络RTK技术并结合测量型天线和接收机，为高精度测量用户提供实时厘米级测量服务。随着物联网、5G等技术的发展，实时分米到亚米级的定位需求逐渐增多。原有的低成本GNSS定位技术无法达到这一精度要求，高精度定位的设备成本高昂，无法用于大众导航领域。因此，本文实现了一种基于CORS系统的低成本差分定位终端。该终端包括两个部分：外接设备和手机。试验表明，在接入CORS系统后，该设备水平方向的定位精度可以达到亚米级，对推进CORS系统的大范围应用具有一定的意义。  相似文献   

基于新型单相MICP技术改性黏性土力学特性的试验研究     

张宽  唐朝生  刘博  张天生  程青  施斌 《工程地质学报》2020,28(2):306-316

微生物诱导碳酸钙沉积(MICP)是一种绿色低碳的新型土体改性技术.该技术当前主要适用于渗透性较好的砂土,普遍使用两相处理方法,即菌液和胶结液分开施用.然而,对于渗透性相对较差的黏性土,传统的两相处理方法难以适用.为此,引入新的单相胶结方法,即菌液和胶结液混合施用,通过调节溶液的初始pH值为细菌水解作用提供窗口期,避免微生物絮凝阻塞孔隙,使混合液均匀分布于土体一定深度范围内,从而达到显著提升胶结效果的目的.利用喷洒法将混合液喷洒至土体表层进行MICP处理,处理完成后使用超微型贯入仪SMP-1测试土体表层不同深度处的结构强度,分析土体力学特性的空间差异,对土体的胶结效果进行定量评价.此外,探究了胶结液浓度(0.2M、0.5M和1.0M)及胶结方法(调节pH与否)对于土体结构强度及MICP改性效果的影响.结果表明:采用单相MICP技术对黏性土进行改性,能够显著提高其结构强度,具有较好的适用性;在不高于1.0 M的胶结液浓度范围内,黏性土的胶结效果随着胶结液浓度增加而提升;相比较而言,调节pH的单相胶结方法对于提升土体胶结的深度和均匀性有明显积极作用.新型单相MICP技术简单易行,能够节约成本,在黏性土表层加固方面具有潜在推广应用价值.  相似文献   

基于岩体塑性位错理论的龙门山区域构造系统演化过程     

师皓宇  黄辅琼  马念杰  王永建  马骥  邹光华  彭瑞 《地质学报》2020,94(12):3581-3589

青藏高原对四川盆地的挤压作用导致了龙门山断裂带的形成及其山脉的隆起。本文以龙门山附近区域板块运动以及深部岩体力学特性为背景，采用FLAC3D软件模拟再现了时间跨度为700万年的龙门山区域构造系统演化过程。研究结果表明：在板块运动作用下，F1、F2和F3断层依次形成，贯通的断层对地表的抬升具有较强的控制作用，当断层贯通于地表后，龙门山及其以西的川西高原持续隆起，平均抬升速率约为138mm/yr，而龙门山断裂带以东的川西坳陷只有较小的抬升量，从而导致川西高原抬升8996 m, 致使该区域产生6000m左右的落差，模拟的地形特征与目前的龙门山地形地貌基本相似。依据模拟结果与实测资料，绘制了龙门山断裂带形成及其附近区域地形地貌的演化过程图，呈现了板块挤压、断层塑性位错、地表侵蚀和沉积作用等因素共同作用对地形地貌的塑造过程。  相似文献   

Acoustic emission characteristics of a dry sandy soil subjected to drained triaxial compression     

Lin  Wenli  Liu  Ang  Mao  Wuwei  Koseki  Junichi 《Acta Geotechnica》2020,15(9):2493-2506

Acoustic emission (AE) technique that is capable of diagnosing the failure process of stressed materials has rarely reported its application to sandy soils subjected to triaxial compression. In this paper, drained triaxial compression tests incorporating with a high-performance AE measurement system were conducted for dry sands with different confining stresses and initial relative densities. Generally, an increased confining stress or initial relative density generates more acoustic emissions, while there also exist exceptions due to different failure patterns. A good resemblance between stress–strain and AE hit rate–strain relations was observed, and power functions between the mechanical parameters and AE hit rate were well established regardless of different confining stresses and initial relative densities. Besides, the behavioral state of yield and peak during compression could be also evaluated by AE hit rate, compared with conventional stress–strain determination. Particularly, the peak AE hit rate is found not always synchronous to but fluctuating at around the peak stress depending on different failure patterns, which might provide beneficial insights into the incipient failure of sands. The present good consistencies suggest that AE characteristics could be used as alternative parameters to evaluate and even predict the mechanical behavior of dry sands.

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以PAC为原料合成锂吸附剂的工艺研究          下载免费PDF全文

张文丁  邓小川  朱朝梁  樊发英  张毅  樊洁  史一飞  卿彬菊 《盐湖研究》2019,27(3)

研究了聚合氯化铝（PAC）为原料合成锂吸附剂的基本工艺路线，并探究了加料方式、反应物配比、反应温度、反应时间和加料速度等对所合成吸附剂吸附量的影响，研究结果表明配比是影响吸附剂吸附量的最主要因素，得到了最佳合成条件，在此条件下所合成吸附剂对锂离子的吸附容量为6.1mg/g。推断吸附剂的主要成分为晶态或非晶态的LiCl·2Al(OH)3·nH2O，同时含有Al(OH)3、(CaO)3Al2O3·6H2O及 LiCl·H2O等成分。从吸附结果来看，结晶度较差的片状LiCl·2Al(OH)3·nH2O和非晶态的Al(OH)3为主成分的吸附剂吸附性能最好。  相似文献   

海洋油气田水下设施的防渔业损坏          下载免费PDF全文

邓周荣  王勇  龙鲲鹏  石锦坤 《海洋开发与管理》2019,36(2):65-67

为保障海洋油气开采的安全和顺利,文章以我国南海东部海域为例,在概述渔场和渔船基本情况的基础上,分析拖网对海洋油气田水下设施可能造成的损坏,并提出相关建议。研究结果表明:对海洋油气田水下设施影响最大的捕捞作业方式是拖网;双拖网渔船产生的最大拖曳力远大于脐带缆所能承受的最大拉力,很有可能损坏脐带缆等水下设施;应对渔船作业水域的海洋油气田水下设施采取保护措施,限制可能损坏水下设施的渔船作业,加强管理和培训以及通过技术手段对相关情况进行监测和处理。  相似文献